DNA Strider was a popular DNA analyze software for macos9. Many professor and student stick to it and generate a lots of DNA sequence file on it.
However, Mac decide to upgrade their os to OSX and lepard and decide not to support classic os9 in intel based MacOSX and intel PowerPC based Lepard anymore. DNA Strider user are stacked and DNA Strider is not under development anymore.
This project is build to help DNA Strider user to get their data out and perform some basic DNA analyze function. It will be able to run on MacOSX, Lepard, Window XP, Windows Vista and Linux.
The program language is Real Basic. It plan to be able to import classic DNA Strider 1.2.1 file and be able to save as new format or export to VectorNTI archive file. More DNA sequence file format is planed to be supported. User will be able to color label selected DNA sequence and translate selected DNA sequence to protein sequence in three different reading frame. User will also be able to do restriction analysis on selected DNA sequence. The software will also provide cloning plan suggestions. Cloning oligo with linker sequence suggestions, site direct mutegenesis suggestions, silent mutation suggestions. The restriction analysis will based on NEB enzyme system, it will provide restriction digest buffer suggestion.
The program is current under development, the current version 1.57 will be able to import classic DNA Strider 1.2.1 file and be able to save as new format to color label selected DNA sequence. Fasta format support is added. File can be export to VectorNTI achive and can be opened by VectorNTI demo version. (You can basically do anything with VectorNTI demo version except paste sequence when you create new DNA sequence. You can bypass the limitation by paste your sequence into DNA strider, export to VectorNTI achive, and import into VectorNTI)
You can do some basic DNA analysis including translate selected DNA sequence to protein sequence in three different reading frame, Restriction summary. More function are coming…
Yizhou Joseph He
University of North Carolina at Chapel Hill
Department of Genetics and Biochemistry and Biophysis